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N/A N=12 Diagnostic

Development of a Skin Test for the Na-ASP-2 Hookworm Antigen

Hookworm Infection · Allergy

Enrolled (actual)
12
Serious AEs
0.0%
Results posted
Feb 2012
Primary outcome: Primary: 100 Mcg/ml Na-ASP-2 Prick-puncture Skin Test — 0 Units on a scale

Study Design & Population

Study type
Interventional
Phase
N/A
Interventions
Na-ASP-2 Skin Test Reagent (Biological)
Age
Adult · 18+ yrs
Sex
All
Sponsor
Albert B. Sabin Vaccine Institute
Primary completion
May 2008

Outcome Measures

OutcomeResultp-value
PRIMARY
100 Mcg/ml Na-ASP-2 Prick-puncture Skin Test
SECONDARY
1000 Mcg/ml Na-ASP-2 Prick-puncture Skin Test
0.21
SECONDARY
100 Mcg/ml Na-ASP-2 Intradermal Skin Test
0.17
SECONDARY
1000 Mcg/ml Na-ASP-2 Intradermal Skin Test
1.25

Summary

Na-ASP-2 is a protein expressed during the larval stage of the N. americanus hookworm life cycle. In a clinical study in previously hookworm-infected adults in Brazil, this protein induced urticarial reactions (rash) in a subset of volunteers. This study will evaluate solutions of varying concentrations of the protein in both a skin prick-puncture and intradermal test that will eventually be used to screen volunteers living in hookworm endemic areas who are being considered as potential participants in vaccine trials, to reduce the potential of inducing allergic reactions in those vaccinated with the Na-ASP-2 Hookworm Vaccine.

Eligibility Criteria

Inclusion Criteria

  • Males or females between 18 and 45 years of age, inclusive.
  • Good general health as determined by means of the screening procedure.
  • Willingness to participate in the study as evidenced by signing the informed consent document.

Exclusion Criteria

  • History of previous infection with hookworm.
  • Prior extensive and continuous travel, work, or residence (> 1 month) in a hookworm-endemic region.
  • Pregnancy as determined by a positive urine hCG test (if female).
  • Evidence of clinically significant neurologic, cardiac, pulmonary, hepatic, rheumatologic, autoimmune, or renal disease by history or physical examination.
  • Behavioral, cognitive, or psychiatric disease that in the opinion of the investigator affects the ability of the volunteer to understand and cooperate with the study protocol.
  • Other condition that in the opinion of the investigator would jeopardize the safety or rights of a volunteer participating in the trial or would render the subject unable to comply with the protocol.
  • Participation in an investigational vaccine or drug trial within 14 days of starting this study.
  • Volunteer has had medical, occupational, or family problems as a result of alcohol or illicit drug use during the past 12 months.
  • History of a severe allergic reaction or anaphylaxis.
  • Known immunodeficiency syndrome.
  • Use of corticosteroids (excluding topical or nasal) or immunosuppressive drugs within 30 days of starting this study.
  • History of a surgical splenectomy.
  • Extensive dermatitis precluding skin testing
  • Current use of a beta blocker (oral or topical) or anti-histamine medication. A volunteer may participate in the study if they agree to withhold use of an anti-histamine for at least 5 days prior to application of the skin test.
  • Use of a tricyclic anti-depressant within the past month.
View full record on ClinicalTrials.gov →

Data sourced from ClinicalTrials.gov (NCT00603889). Outcome figures and adverse-event rates are extracted automatically from the registry's posted results and are provided for clinician reference, not as a substitute for the primary publication.

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