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Phase 1 N=5 Treatment

Study of Donor Derived, Multi-virus-specific, Cytotoxic T-Lymphocytes for Relapsed/Refractory Neuroblastoma

Neuroblastoma

Enrolled (actual)
5
Serious AEs
100.0%
Results posted
Jul 2019
Primary outcome: Primary: Number of Participants With Immediate and Short Term Toxicity of Infusion Over 8 Weeks — 0; 0; 3 Participants

Study Design & Population

Study type
Interventional
Phase
Phase 1
Interventions
GD2 CAR modified Tri-virus specific cytotoxic t-cells (Biological)
Age
Pediatric · 0+ yrs
Sex
All
Sponsor
Children's Mercy Hospital Kansas City
Primary completion
Jan 2015

Outcome Measures

OutcomeResultp-value
PRIMARY
Number of Participants With Immediate and Short Term Toxicity of Infusion Over 8 Weeks
0; 0; 3
PRIMARY
Peak Transgene Copy Number Per 1000ng PBMC DNA
10562; 44; 1464
PRIMARY
Death Within 8 Weeks of Infusion
1
SECONDARY
Peak Viral Specific SFU/2x10e5 Mononuclear Cells Per Well
180; 175; 150
SECONDARY
Maximum Tumor Response (RECIST 1.1)
0; 3; 0

Summary

This is a single-center, investigator-initiated, single-arm, pilot study of post-allogeneic transplant, adoptive immunotherapy for the treatment of patients with relapsed/refractory neuroblastoma expressing the mesenchymal tumor marker GD2. Three patients will be treated. The study will focus on the safety and efficacy of allogeneic, donor derived viral specific cytotoxic T-lymphocytes, retrovirally transduced to express a chimeric antigen receptor specific for disialoganglioside, GD2, expressed on neuroblastoma.

Eligibility Criteria

Inclusion Criteria

  • Allogeneic transduced tV-CTLs with >15% expression of 14g2a.zeta chimeric antigen receptor
  • Patient or responsible person must be able to understand and sign a permission/assent or consent form for infusion
  • Age 18 months through 17 years at time of relapse/progression
  • Life expectancy >8weeks
  • Karnofsky score 60% or greater if 10 yrs old or older. Lansky score 60% or greater if under 10 yrs old
  • Patient must be HIV negative
  • ANC >500
  • Pulse ox>90% on room air
  • AST/ALT/direct bili 50% donor engraftment

Exclusion Criteria

  • Patient pregnant or lactating or refuses birth control methods
  • HIV positive
  • Uncontrolled intercurrent infection
  • Renal failure (creatinine clearance 5xnormal
  • Rapidly progressive disease
  • Currently receiving any investigational drugs
  • Tumor potentially causing airway obstruction
  • Cardiomegaly or bilateral pulmonary infiltrates on CXR
  • Receiving >0.25mg/kg/day methylprednisolone or equivalent systemic steroid. Topical steroid therapy is acceptable
  • Receiving more than one lymphocyte inhibiting agent (ex. Tacrolimus/CSA and MMF or other similar agent
  • Patients relapsing or progressing before the age of 18 months from Stage I/II disease, and/or those who, in the opinion of their oncologist, may benefit from further conventional therapy
  • Donor lymphocyte infusion in last 28 days
  • Evidence of GvHD greater than or equal to grade 2
View full record on ClinicalTrials.gov →

Data sourced from ClinicalTrials.gov (NCT01460901). Outcome figures and adverse-event rates are extracted automatically from the registry's posted results and are provided for clinician reference, not as a substitute for the primary publication.

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