Safety and Immunogenicity of Prime-Boost Vesicular Stomatitis Virus (VSV) Ebola Vaccine in Healthy Adults (V920-002)

INCLUSION CRITERIA

• Healthy male or females, ages 18 to 65 (inclusive) at the time of screening
• Females of childbearing potential and all males, must be willing to use effective methods of contraception, from at least 14 days prior to vaccination through Day 56 which would include:
• Oral contraceptives, either combined or progestogen alone
• injectable progestogen
• implants of etonogestrel or levonorgestrel
• oestrogenic vaginal ring
• percutaneous contraceptive patches
• intrauterine device or intrauterine system
• male partner sterilization
• male condom combined with a spermicide
• Must be willing to minimize blood and body fluid exposure of others for at least 7 days after vaccination, which includes:
• Use of effective barrier prophylaxis, such as latex condoms, during any sexual interaction (regardless of childbearing status or sexual orientation)
• Avoiding the sharing of needles, razors, eating utensils, drinking from the same cup, or toothbrushes
• Avoiding open-mouth kissing
• Must be willing to forgo blood donation for one year
• Must agree not to enroll in another study of an investigational agent prior to completion of Day 56 and not participate in an investigational vaccine study until the last required protocol visit on Day 365
• Ability to provide informed consent

EXCLUSION CRITERIA

FACTORS THAT INCREASE RISK TO THE SUBJECT:

• Clinically significant medical condition, physical examination findings, clinically significant abnormal laboratory results, or past medical history with clinically significant implications for current health. A clinically significant condition or process includes but is not limited to:
• A process that would affect the immune response
• A process that would require medication that affects the immune response
• Any contraindication to repeated injections or blood draws
• A condition that requires active medical intervention or monitoring to avert grave danger to the participant's health or well-being during the study period
• A condition or process for which signs or symptoms could be confused with reactions to vaccine
• Any condition specifically listed among the exclusion criteria below
• Active malignancy
• Asplenia
• History of Guillain-Barré Syndrome
• History of neurological or neuropsychiatric disorder that may either increase risk (history of encephalitis, narcolepsy, stroke, depression, bipolar disorder, seizure, etc.) or could interfere with the assessment of safety (e.g., frequent headaches)
• History of autoimmune disease
• History of hemoglobinopathy or a coagulopathy
• Women who are breast-feeding
• Positive urine or serum pregnancy test
• Abnormal chemistry panel; defined as:
• Defined as any Grade 3 or greater toxicity (regardless of clinical significance) by the toxicity table
• Evaluating only creatinine, alanine aminotransferase, aspartate aminotransferase, total bilirubin, and estimated glomerular filtration rate
• Abnormal complete blood count (CBC) defined as:
• Defined as any Grade 3 or greater toxicity (regardless of clinical significance) by the toxicity table
• Evaluating only the white blood cell (WBC), hemoglobin, hematocrit, and platelets
• Abnormal urinalysis defined as:
• Defined as any Grade 3 or greater toxicity (regardless of clinical significance) by the toxicity table
• Evaluating red blood cells (RBC), protein, and glucose only
• Positive serology for hepatitis B surface antigen
• Positive serology for hepatitis C
• Positive serology for human immunodeficiency virus (HIV)
• Known allergy to the components of the VSVΔG-ZEBOV vaccine (V920) vaccine product (VSV, albumin, tris)
• History of severe local or systemic reactions to any vaccination or a history of severe allergic reactions

FACTORS THAT MAY LIMIT VSV REPLICATION OR MAKE INTERPRETATION OF IMMUNOGENICITY DIFFICULT:

• History of prior infection with a filovirus or prior participation in a filovirus vaccine trial
• Veterinarian or ranchers exposed to livestock known to be infecte