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N/A N=121 Randomized Triple-blind Basic Science

Somatosensory Modulation of Salivary Gene Expression and Oral Feeding in Preterm Infants

Infant, Extremely Low Birth Weight · Feeding Behavior

Enrolled (actual)
121
Serious AEs
0.0%
Results posted
Feb 2026
Primary outcome: Primary: Salivary Gene Expression — 21.344; 21.420; 20.931; 21.100 Delta-CT — p=<0.01

Study Design & Population

Study type
Interventional
Phase
N/A
Interventions
NTrainer (Device); SHAM blind pacifier (Device)
Age
Pediatric
Sex
All
Sponsor
University of Nebraska Lincoln
Primary completion
May 2021

Outcome Measures

OutcomeResultp-value
PRIMARY
Salivary Gene Expression
21.344; 21.420; 20.931; 21.100; 11.879; 11.844 <0.01 sig
SECONDARY
NNS Bursts/Minute
5.72; 5.98; 5.71; 5.21 <0.05 sig
SECONDARY
Time-to-transition to Full Oral Feed
17.40; 12.79; 15.24; 7.13 <0.05 sig
SECONDARY
NNS Cycles/Min
54.56; 53.70; 55.89; 50.30 <0.05 sig
SECONDARY
Non-nutritive Suck Spatiotemporal Index (NNS STI)
63.84; 65.16; 65.97; 67.62 <0.05 sig
SECONDARY
Non-nutritive Suck Compression Amplitude (cmH2O)
28.41; 27.87; 29.03; 22.68 <0.05 sig
SECONDARY
National Institute Child Human Development (NICHD) Neonatal Research Network Feed-growth Questionnaire
8; 3; 11; 5; 4; 3 <0.05 sig
SECONDARY
Bayley III Developmental Scales-Cognition
91.53; 91.41; 88.06; 86.23 0.7989
SECONDARY
Bayley III Developmental Scales-Language
86.22; 88.95; 83.73; 85.50 0.8979
SECONDARY
Bayley III Developmental Scales-Motor
90.95; 96.41; 89.89; 85.30 0.1085

Summary

Two innovative approaches, pulsatile orocutaneous entrainment of non-nutritive suck via orosensory entrainment (NTrainer) device technology and serial salivary gene expression analyses, will be merged to examine the relation between gene expression, oral somatosensory stimulation, feeding behavior, and neurodevelopmental outcomes at 18 months corrected age (CA) on 180 extremely preterm infants [EPIs] (24 0/7-26 6/7 GA and 27 0/7 - 28 6/7 GA) enrolled at three neonatal intensive care units: Catholic Health Initiative (CHI) Health St. Elizabeth (Lincoln, NE), Tufts Medical Center (Boston, MA), and Santa Clara Valley Medical Center (San Jose, CA). EPIs will be randomized to a blind pacifier (SHAM) or PULSED NTrainer treatment groups, and stratified by GA, sex, and bronchopulmonary dysplasia status (BPD vs non-BPD). We hypothesize that the combination of the NTrainer® intervention for improved oral feeding skills, along with objective salivary gene expression data to monitor response to treatment and feeding development, will result in a novel, objective, and personalized approach to neonatal oral feeding and reduce the duration of time to attain oral feeds while improving feeding, growth and neurodevelopmental outcomes at 18 months' CA.

Eligibility Criteria

Inclusion Criteria

  • Extremely preterm infants (EPIs) born between 24 0/7 and 28 6/7 weeks' GA, as determined by obstetric ultrasound at 90th percentile
  • Intracranial hemorrhage grades III and IV, seizures
  • Meningitis
  • Neurological examination showing abnormal tone or movements of all extremities for PCA
  • History of necrotizing enterocolitis (stage II and III)
  • Culture-positive sepsis at the time of study enrollment
View full record on ClinicalTrials.gov →

Data sourced from ClinicalTrials.gov (NCT02696343). Outcome figures and adverse-event rates are extracted automatically from the registry's posted results and are provided for clinician reference, not as a substitute for the primary publication.

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