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Phase 4 N=20 Randomized Quadruple-blind Basic Science

Defibrotide in the Human Endotoxemia Model --- an Exploratory Trial Investigating the Effects and the Mechanisms of Defibrotide

Healthy Volunteers · Endotoxemia

Enrolled (actual)
20
Serious AEs
0.0%
Results posted
Dec 2019
Primary outcome: Primary: Prothrombin Fragments f1+2 — 31.1; 34.08; 27.41; 36.02 fold-change*h — p=0.408

Study Design & Population

Study type
Interventional
Phase
Phase 4
Interventions
Defibrotide (Drug); Placebo (0.9% sodium chloride) (Drug); Lipopolysaccharide (Drug); Placebo (0.9% sodium chloride bolus) (Drug)
Age
Adult, Older Adult · 18+ yrs
Sex
All
Sponsor
Bernd Jilma
Primary completion
Feb 2018

Outcome Measures

OutcomeResultp-value
PRIMARY
Prothrombin Fragments f1+2
31.1; 34.08; 27.41; 36.02 0.408
SECONDARY
Thrombin-Antithrombin Complexes
23.39; 27.4; 17.46; 21.83 0.148
SECONDARY
Plasmin-Antiplasmin Complexes
11.8; 9.99; 7; 7.12 0.039 sig
SECONDARY
Tumor Necrosis Factor (TNF)-Alpha
286; 326; 28.08; 21.01 0.642
SECONDARY
Tissue-type Plasminogen Activator
21.8; 17; 20.57; 23.54 0.026 sig
SECONDARY
Interleukin-6
1543; 1144; 35; 31 0.326
SECONDARY
E-Selectin
35; 33; 21; 41 0.796
SECONDARY
Plasminogen Activator Inhibitor 1
216; 477; 25; 24.86 0.918
SECONDARY
Von Willebrand Factor Antigen
54; 53; 27; 26 0.877
SECONDARY
Clotting Time in Thromboelastometry
1.12; 1.12; -0.2; 2.14 0.423
SECONDARY
Maximum Lysis in Thromboelastometry
7.9; 7.7; 6.84; 6.85 0.938

Summary

Defibrotide is an anti-inflammatory and anti-coagulatory agent approved for treatment of veno-occlusive disease. Although it has been in clinical use for almost 30 years, the exact mechanism of action has never been fully elucidated. Thus, the effects of defibrotide will be investigated in the human endotoxemia model in order to gather further information on its actions.

Eligibility Criteria

Inclusion Criteria

  • >18 years of age
  • <90kg body weight
  • Normal findings in medical history and physical examination unless the investigator considers the abnormality to be clinically irrelevant
  • Normal laboratory values unless the investigator considers abnormalities to be clinically irrelevant
  • Ability to understand the purpose and nature of the study, as well as the associated risks

Exclusion Criteria

  • Intake of any drugs that may interfere with the trial's endpoints or drugs (i.e. platelet inhibitors, anticoagulants, etc.)
  • Positive results of HIV or hepatitis virology
  • Acute illness with systemic inflammatory reactions
  • Known allergies, hypersensitivities or intolerances to any of the used substances
  • Acute or recent bleeding episodes, increased risk of bleeding at the discretion of the investigator
  • Participation in an LPS trial within 6 weeks of the first study day
  • Pregnancy or breastfeeding
View full record on ClinicalTrials.gov →

Data sourced from ClinicalTrials.gov (NCT02876601). Outcome figures and adverse-event rates are extracted automatically from the registry's posted results and are provided for clinician reference, not as a substitute for the primary publication.

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