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N/A N=52 Randomized Basic Science

Milk in Life Conditions (MiLC): Bacterial Composition of Human Milk Pumped and Stored in "Real-Life" Conditions

Human Milk Microbiome · Bacterial Growth

Enrolled (actual)
52
Serious AEs
Results posted
May 2019
Primary outcome: Primary: Bacterial Community Richness — 64.2; 58.9; 64.0; 60.8 Observed taxa — p=0.9

Study Design & Population

Study type
Interventional
Phase
N/A
Interventions
Sterile pump set-up (Device); Mother's Own pump set-up (Device)
Age
Adult, Older Adult · 18+ yrs
Sex
Female
Sponsor
Cornell University
Primary completion
Oct 2017

Outcome Measures

OutcomeResultp-value
PRIMARY
Bacterial Community Richness
64.2; 58.9; 64.0; 60.8 0.9
PRIMARY
Bacterial Community Diversity
3.89; 3.56; 3.84; 3.93 0.3
PRIMARY
Total Live Aerobic Bacterial Counts
1848; 1233; 9157; 6109 0.0003 sig

Summary

The MiLC trial is a randomized control trial of two different breast pump set-ups: mother's own and sterile. The objective of this trial is to investigate the bacterial composition of human milk pumped and stored in "real-life" conditions. To meet this objective, lactating mothers will fully express breast milk from one breast on two consecutive pumping sessions at home, once with the participant's own pumps and collection kits (own pump set-up) and once with a hospital-grade pump and disposable, sterile collection kits (sterile pump set-up). Randomization will be used to determine which pump participants use first. From the total volume of milk pumped during each pumping session, the researchers will collect 1 oz. Milk from both pumps will be stored at home and sampled on days 0, 2, 4, and 30 after expression for analysis of its bacterial composition.

Eligibility Criteria

Inclusion Criteria

  • Lactating women over the age of 18 years
  • Self-reported as healthy women and infants
  • Use an electric breast pump
  • Confident of ability to donate 1 oz of milk from one breast during each of two consecutive pumping sessions where pumping sessions are 3 hours (+/- 30 minutes) apart and between 0700-1100 hours.
  • Able to store donated milk at home for 30 days
  • Have infants who do not consume formula or only consume formula episodically as long as the most recent formula-feeding occurred > 2 weeks before the day milk is pumped for this study.

Exclusion Criteria

  • Not confident of ability to donate 1 oz of milk from one breast during each of two consecutive pumping sessions, 3 hours (+/- 30 minutes) apart and between the hours of 0700-1100 hours.
  • Infant consumption of formula in the past 2 weeks
  • Current indication of breast infection (e.g., breast pain, discomfort, lumps, mastitis with fever, red streaks, or hard red portions of the breast)
  • Breast pain that the woman does not consider "normal" for lactation/breastfeeding
  • Signs/symptoms of acute illness in woman or infant in past 7 days including fever (rectal or temporal temperature ≥99.5 F), dark green nasal discharge, diarrhea (abrupt onset of 3 or more excessively "loose" stools in one day), vomiting (where infant vomiting is not associated with feeding), or severe cough.
View full record on ClinicalTrials.gov →

Data sourced from ClinicalTrials.gov (NCT03123874). Outcome figures and adverse-event rates are extracted automatically from the registry's posted results and are provided for clinician reference, not as a substitute for the primary publication.

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