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Phase 3 N=222 Randomized Single-blind Treatment

Adjusted Fibrinogen Replacement Strategy

Bleeding Disorder · Hypofibrinogenemia; Acquired

Enrolled (actual)
222
Serious AEs
31.1%
Results posted
Jun 2025
Primary outcome: Primary: Intra-operative Blood Loss — 1380.7; 1660.13 mL — p=<0.001

Study Design & Population

Study type
Interventional
Phase
Phase 3
Interventions
BT524 (Biological); FFP/Cryo (Biological)
Age
Adult, Older Adult · 18+ yrs
Sex
All
Sponsor
Biotest
Primary completion
Sep 2023

Outcome Measures

OutcomeResultp-value
PRIMARY
Intra-operative Blood Loss
1380.7; 1660.13 <0.001 sig
SECONDARY
Proportion (%) of Subjects With Successful Correction of Fibrinogen Level (FIBTEM A10) 15 Minutes After Start of First IMP Administration
59; 19 <0.001 sig
SECONDARY
Time to First Successful Correction of Fibrinogen Level
59; 19; 17; 11; 11; 16 <0.001 sig
SECONDARY
Transfusion Requirements: Cell Salvage
95.6; 78.0
SECONDARY
Transfusion Requirements: Allogeneic Platelets
3.0; 3.6
SECONDARY
Transfusion Requirements: Allogeneic Red Blood Cells
455.5; 488.4
SECONDARY
Transfusion Requirements: Fresh Frozen Plasma
60.5; 14.8
SECONDARY
Transfusion Requirements, Cryoprecipitate
0; 0
SECONDARY
Amount of Red Blood Cells (RBCs)
543.4; 558.9 <0.831
SECONDARY
Post-operative Blood Loss
306.3; 293.9 =0.809
SECONDARY
Subjects With Rebleeds
0; 5 =0.022 sig
SECONDARY
Hospital Length of Stay After Surgery
7; 4; 4; 10; 13; 12
SECONDARY
In-hospital Mortality
0; 0
SECONDARY
Number of Subjects With Thrombosis or Thromboembolic Events (TEEs)
8; 13
SECONDARY
Change in Viral Status
0; 0

Summary

The main purpose of this study was to demonstrate the efficacy and safety of intraoperative use of fibrinogen concentrate BT524, as a complementary therapy for the management of uncontrolled severe hemorrhage in acquired hypofibrinogenemia. This non-inferiority study focused on the primary objective of demonstrating that BT524 is non-inferior that means not worse than the comparator fresh frozen plasma/cryoprecipitate in reducing intraoperative blood loss when administered intravenously in subjects with acquired hypofibrinogenemia undergoing elective major spinal or abdominal surgery.

Eligibility Criteria

Inclusion Criteria

At screening:

  • Written informed consent
  • Subjects scheduled for elective major spinal surgery or cytoreductive pseudomyxoma peritonei (PMP) surgery with expected major blood loss
  • Male or female, aged ≥ 18 years
  • No increased bleeding risk as assessed by standard coagulation tests and medical history

Intra-operative:

5.

  • Subjects who underwent spinal surgery: Intra-operative clinically relevant bleeding of approximately 1 Liter, requiring hemostatic treatment during surgery.
  • Subjects who underwent cytoreductive PMP surgery: Intra-operative prediction of clinically relevant bleeding of more than 2 Liter, requiring hemostatic treatment during surgery

Exclusion Criteria

  • Pregnancy or unreliable contraceptive measures or breast feeding (women only)
  • Hypersensitivity to proteins of human origin or known hypersensitivity reactions to components of the Investigational Medicinal Products (IMP)
  • Participation in another clinical study within 30 days before entering the study or during the study and/or previous participation in this study
  • Treatment with any fibrinogen concentrate and/or fibrinogen-containing product within 30 days prior to infusion of IMP
  • Employee or direct relative of an employee of the Contract Research Organization (CRO), the study site, or Biotest
  • Inability or lacking motivation to participate in the study
  • Medical condition, laboratory finding (e.g., clinically relevant biochemical or hematological findings outside the normal range), or physical exam finding that in the opinion of the investigator precludes participation
  • Presence or history of venous/arterial thrombosis or thromboembolic event (TEE) in the preceding 6 months
View full record on ClinicalTrials.gov →

Data sourced from ClinicalTrials.gov (NCT03444324). Outcome figures and adverse-event rates are extracted automatically from the registry's posted results and are provided for clinician reference, not as a substitute for the primary publication.

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