Sleep Disruption Induced Impairments in Bone Formation

Inclusion Criteria

o Adult men aged 20-65 years old who habitually sleep 7-9 hours/night.

Exclusion Criteria

• Regularly go to sleep after midnight;
• Shift work 1 year prior to study;
• Travel >1 time zone 4 weeks prior to study or need to travel during study;
• More than moderate activity level (>3 days of exercise per week >30 min of exercise per session);
• Current smokers (or within the previous year of study);
• Positive drug test at screening or laboratory admission;
• BMI > 30 kg/m2;
• Individuals who are concurrently participating in another research protocol that would influence their safe participation in this study. For example, participants involved in a study that requires blood draws or ingestion of experimental medication as this would increase the risk of participation in our study and/or compromise study results.
• Any clinically significant unstable medical or surgical condition within the last year (treated or untreated), including history of a clinically significant abnormality of the neurological system (including cognitive disorders or significant head injury) or any history of seizure (including febrile seizure-sleep loss has been used clinically to induce seizures in patients with epilepsy). Given the wide range of illnesses that are encountered in medical practice, it would not be possible to provide a comprehensive list of each and every disease that could serve as grounds for exclusion for the subject. However, the following is a list of illness categories that would certainly be grounds for exclusion: Connective Tissue and Joint Disorders; Neurologic/cognitive Disorders; Musculoskeletal Disorders; Immune Disorders; Chronobiologic Disorders; Cardiovascular Disorders; Respiratory Disorders; Kidney Disorders; Infectious Diseases; Hematopoietic Disorders; Neoplastic Diseases; and Endocrine and Metabolic Diseases.
• Self-reported or newly diagnosed medical condition that is still being investigated or is not under good control, including those identified on screening labs such as:
• Out-of-range values measured on a fasting blood sample: glucose > 100 mg/dl, thyroid stimulating hormone 5.0 uU/ml, abnormal alkaline phosphatase 117 U/l, creatinine, or hemoglobin 500 mg/day) or alcohol use (>14 standard drinks/week or >5 drinks in one sitting);
• Subjects with a history of heparin-induced thrombocytopenia (HIT) or an allergy to heparin;
• Inability to travel to the CU-AMC campus for study visits.
• Individuals with restrictive diets (e.g., vegan)
• Individuals with 25OHD < 20 ng/mL;
• Individuals with eGFR < 60 mL/min/1.73m2 as this is known to affect CTX measurements;
• T-score ≤ -2.5 (men ≥50 years old) or Z-score < -2.0 (men <50 years old) for bone mineral density (BMD) at the L-spine, femoral neck, or total hip on baseline DXA as compared to the DXA machine's normative database;
• Symptoms of active illness (e.g., fever); note that subject can be studied at a later date.