Evaluating the Comparative Pharmacokinetics of Nicotine After Administration Via JUUL or Tobacco Cigarettes

Inclusion Criteria

• Healthy on the basis of medical history and limited physical examination, as described below:
• - Heart rate 90*
• - Diastolic Blood Pressure 50*
• Age: >= 21 & 0 mg/ml
• Group 1 Experienced E-cigarette users
• - Current use of tobacco cigarettes ( = 5 cigarettes per day
• - Current e-cigarette use must be =50 ng/ml or urine cotinine and/or NicAlert=6
• Must have a smart phone, computer, or tablet and internet access (for remote procedures)

Exclusion Criteria

• Medical
• - Heart disease
• - Seizures
• - Cancer
• - Thyroid disease (okay if controlled with medication)
• - Diabetes
• - Hepatitis B or C or Liver disease
• - Glaucoma
• - Kidney disease or urinary retention
• - History of stroke
• - An ulcer in the past year
• - Active use of an inhaler for Asthma or Chronic Obstructive Pulmonary Disease (COPD)
• Psychiatric conditions
• - Current or past schizophrenia, and/or current or past bipolar disorder
• - Major depression, current or within the past year
• - Major personality disorder
• - Participants with current or past minor or moderate depression and/or anxiety disorders will be reviewed by the principal investigator (PI) and/or medical monitor and considered for inclusion
• - History of psychiatric hospitalizations are not exclusionary, but study participation will be determined as per PI's or medical monitor's approval
• Drug/Alcohol Dependence
• - Alcohol or illicit drug dependence within the past 12 months with the exception of those who have recently completed an alcohol/drug treatment program
• - Positive toxicology test for illicit drugs at the screening visit (Tetrahydrocannabinol (THC) & prescribed medications okay)
• - Opioid replacement therapy (including methadone, buprenorphine, or other)
• Psychiatric medications
• - Current regular use of any psychiatric medications is exclusionary, with the exception of Selective Serotonin Reuptake Inhibitor (SSRI) and serotonin-norepinephrine reuptake Inhibitor (SNRI) and current evaluation by the PI and/or medical monitor that the participant is otherwise healthy, stable, and able to participate
• Medications
• - Use of sympatholytic medications for cardiovascular conditions including hypertension (Example: beta and alpha-blockers)
• - Use of medications that are inducers of nicotine metabolizing enzyme CYP2A6 (Example: rifampicin, carbamazepine, phenobarbital, and other anticonvulsant drugs)
• - Concurrent use of nicotine-containing medications
• - Any stimulant medications (example: Adderall) generally given for attention deficit hyperactivity disorder (ADHD) treatment
• Use of Other Tobacco Products (OTP)
• - Any of the following products in combination more than 15 times in the past month
• - - smokeless tobacco (snus, oral snuff, chewing tobacco)
• - - pipes
• - - cigars, cigarillos, little cigars
• - - blunts, spliffs
• - - hookah
• Other/Misc. Chronic Health Conditions
• Fainting (within the last 30 days)
• Other "life threatening illnesses" as per PI's or medical monitor's discretion
• Pregnancy
• - Pregnancy (self-reported and urine pregnancy test)
• - Breastfeeding (determined by self-report)
• Concurrent participation in another clinical trial (at PI's discretion)
• Inability to read and write in English
• Planning to quit smoking or vaping within the next 60 days
• Recent onset or change (worsening) in cough, fever and/or abdominal symptoms (vomiting or pain) in the past two weeks
• Diagnosis of pneumonia in the past 3 months
• Uncomfortable with blood draws
• Known allergy to propylene glycol or vegetable glycerin