N/A N=12 Randomized Other

Impact of Muscle Temperature on Muscle Growth and Breakdown: Cooling During Resistance Training

Exercise Training

Bottom Line

View on ClinicalTrials.gov: NCT05302791 ↗

Enrolled (actual)

Serious AEs

0.0%

Results posted

Apr 2024

Primary outcome: Primary: RT-qPCR for Forkhead Box O3 (FOXO3a) — 1.24; 1.30 fold change — p=.609

Study Design & Population

Study type: Interventional
Phase: N/A
Interventions: Muscle Cooling (Device); Resistance Exercise (Other)
Age: Adult · 19+ yrs
Sex: All
Sponsor: University of Nebraska
Primary completion: Oct 2022

Outcome Measures

Outcome	Result	p-value
PRIMARY RT-qPCR for Forkhead Box O3 (FOXO3a)	1.24; 1.30	.609
PRIMARY RT-qPCR for Tripartite Motif Containing 63 (TRIM63)	3.59; 3.50	.858
PRIMARY RT-qPCR for Growth/Differentiation Factor 8 (MSTN)	.44; .44	.633
PRIMARY RT-qPCR for Myogenic Factor 5 (MYF5)	.51; .83	.076
PRIMARY RT-qPCR for Myogenic Factor 6 (MYF6)	2.2; 1.32	.151
PRIMARY RT-qPCR for Myocyte Enhancer Factor 2A (MEF2a)	1.44; 1.91	.385
PRIMARY RT-qPCR for Myogenic Differentiation 1 (MYOD1)	2.08; 1.92	.665
PRIMARY RT-qPCR for Myogenin (MYOG)	1.88; 2.62	.078
PRIMARY RT-qPCR for Ribosomal Protein S3 (RPS3)	1.46; 1.29	.773
PRIMARY RT-qPCR for Ribosomal Protein L3 Like (RPL3L)	1.55; .78	.118
SECONDARY Muscle Temperature	26.09; 35.07	<0.001 sig
SECONDARY Skin Temperature	20.4; 31.65	<0.001 sig

Summary

The purpose of this study is to examine the effect of human skeletal muscle temperature during resistance exercise on myogenic and proteolytic signaling. Subjects will perform bilateral resistance exercise bouts at an intensity that should stimulate a muscle growth response in the Vastus Lateralis. During the resistance exercise, the subjects will receive a cold (10°C) intervention on the experimental limb, while receiving a neutral temperature intervention (22°C) on the control limb.

Eligibility Criteria

Inclusion Criteria

Any male or female who is 19-45 of age and is considered
low risk for physical activity based on the American College of Sports Medicine (ACSM) guidelines
recreationally active during the 6 weeks prior to participation by meeting the ACSM minimum activity guidelines

Exclusion Criteria

Any person outside of the age range of 19-45
not considered 'low risk' on the ACSM Risk Stratification Form
not meeting the exercise requirements for the preceding 6 weeks will be excluded.
self-report of a history of joint or muscular injury that would prevent them from safely completing the required activities
self-report of taking prescription drugs or supplements that may interfere with this research
self-report allergy to Xylocaine
pregnant
breast feeding
self-report sensitivity to heat or cold

View full record on ClinicalTrials.gov →

Data sourced from ClinicalTrials.gov (NCT05302791). Outcome figures and adverse-event rates are extracted automatically from the registry's posted results and are provided for clinician reference, not as a substitute for the primary publication.