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Extracellular vesicles show consistent presence and biological activity across diverse neonatal conditionsSmall particles called extracellular vesicles show promise in neonatal health

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Key Takeaway
Note that while EVs show consistent activity in neonates, evidence for their use as biomarkers is currently limited.

This systematic review synthesizes findings from 43 predominantly observational studies to evaluate the presence, biological activity, and potential clinical utility of extracellular vesicles (EVs) in term and preterm neonates. The scope includes investigating the pathophysiological roles of EVs and their potential as diagnostic or prognostic biomarkers in neonatal health.

The synthesis indicates a consistent presence and biological activity of EVs across diverse neonatal conditions. While these findings suggest that EVs may function as pathophysiological mediators, the authors note that evidence is currently insufficient to establish definitive functions. The review highlights the potential for EVs to serve as candidate biomarkers in various neonatal conditions.

Several limitations are noted, including low certainty due to heterogeneous methods and small to moderate sample sizes in the included studies. Clinical utility of these markers is not yet established, and standardized studies are required before clinical application can be determined. The evidence remains limited and should be interpreted with caution.

Imagine trying to understand what is happening inside a newborn's body during a critical moment. Doctors are looking for better ways to monitor these tiny patients. A review of 43 studies suggests that small particles called extracellular vesicles (EVs) could play a major role in this process.

These EVs are found consistently in the fluids of both full-term and premature babies across many different conditions. Because they appear so regularly, researchers believe they might act as markers to help identify health issues or predict how a baby's condition might progress.

While these findings are promising, it is important to note that the evidence is still limited. The studies looked at were very different from one another in their methods. Because of this, we cannot yet say for sure exactly what these particles do or how they can be used in a hospital setting just yet.

What this means for you:
Extracellular vesicles are consistently found in newborns and may eventually help doctors track health status.

Common questions

What are extracellular vesicles?

Extracellular vesicles, or EVs, are small particles found in the biological fluids of newborns. This review looked at 43 studies to see how these particles behave. They appear consistently across various conditions in both term and preterm babies, which suggests they might play a role in how the body functions.

Can these particles be used to diagnose health issues in babies?

There is potential for these particles to be used as biomarkers. A biomarker is a measurable sign of a condition. While they are consistently present in neonatal fluids, the evidence is currently limited and more standardized research is needed before they can be used reliably in clinical practice.

Is the evidence for using these particles in newborns certain?

The certainty of these findings is currently low. Because the 43 studies included in the review used very different methods and had small to moderate sample sizes, more research is needed to confirm exactly how these particles function in a clinical setting.

Study Details

Study typeMeta analysis
EvidenceLevel 1
PublishedJun 2026
View Original Abstract ↓
ObjectiveExtracellular vesicles (EVs) are cell-derived, membrane-bound particles that have gained increasing attention for their roles in pathophysiology, diagnostics, and targeted therapies. This review aimed at systematically collecting and critically synthesizing current evidence on EVs derived from neonatal biological fluids, focusing on their involvement in neonatal health and disease and their potential diagnostic and prognostic value.MethodsA systematic literature review was conducted in PubMed and Scopus databases between August and November 2025. The study protocol was registered in PROSPERO (CRD420251151417).ResultsFollowing a PRISMA-compliant search strategy, 43 predominantly observational studies were included, encompassing term and preterm neonates with small-to-moderate sample sizes and heterogeneous clinical settings. EVs were isolated from multiple biological matrices, most frequently umbilical cord and peripheral blood, with sampling mainly performed at birth or during early postnatal life. Overall, the findings demonstrate the consistent presence and biological activity of EVs across diverse neonatal conditions, supporting their potential role as pathophysiological mediators and candidate biomarkers.ConclusionsAlthough EVs appear to actively participate in key neonatal processes, the available evidence remains limited and methodologically heterogenous. Larger, standardized studies are required to clarify their biological functions and clinical utility.Systematic Review Registrationhttps://www.crd.york.ac.uk/PROSPERO/view/CRD420251151417, identifier CRD420251151417.
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