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Phospholipid concentrations in human milk vary across colostrum, transitional, and mature milk stagesPhospholipids in human milk vary by stage and detection method

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Key Takeaway
Note that total phospholipid concentrations in human milk decrease from colostrum to mature milk stages.

This systematic review and meta-analysis included 25 studies to evaluate the concentration of total phospholipids and their subclasses in human milk. The analysis focused on three distinct stages: colostrum, transitional milk, and mature milk. The study also examined the impact of detection methods on reported concentrations.

The meta-analysis of 18 studies identified specific concentrations for total phospholipids: 251.60 mg/L in colostrum (175.62, 327.58), 234.71 mg/L in transitional milk (171.07, 298.35), and 197.00 mg/L in mature milk (166.08, 227.93). The primary phospholipid classes identified were PC, SM, and PE. Furthermore, the analysis noted that detection methods had a significant effect on the reported concentrations of total phospholipids, PE, and PI in mature milk.

These findings provide a theoretical basis for the application of phospholipids in the development of infant formula. No specific limitations or adverse events were reported in the data provided. The results suggest that phospholipid levels are highest in the earliest stages of lactation.

When a baby is first born, the milk they receive is called colostrum. As the baby grows, the milk transitions into mature milk. Scientists looked at 25 different studies to see how phospholipids, which are important fats in the milk, change during these stages. They found that the concentration of these fats is highest in colostrum and decreases as the milk matures.

Specifically, colostrum contains about 251.60 mg/L of total phospholipids, while transitional milk has 234.71 mg/L. By the time it becomes mature milk, the concentration drops to 197.00 mg/L. These findings help researchers understand the nutritional makeup of human milk at every stage of early life.

One important finding is that the way scientists measure these fats in a lab can change the results. Different detection methods significantly affected the reported amounts of total phospholipids and specific types like PE and PI. This research provides a foundation for how these fats might be used in infant formulas.

What this means for you:
Phospholipid levels are highest in colostrum and decrease as human milk matures into the mature stage.

Common questions

How do phospholipid levels change as milk matures?

The concentration of total phospholipids is highest in colostrum at 251.60 mg/L. It drops slightly to 234.71 mg/L in transitional milk and reaches its lowest measured level of 197.00 mg/L in mature milk.

What are the main types of phospholipids found in milk?

The study identified three major phospholipid classes present in the milk: PC, SM, and PE.

Do different testing methods change the results?

Yes, the study found that the specific detection methods used in a lab have a significant effect on the measured concentrations of total phospholipids and specific types like PE and PI.

Study Details

Study typeMeta analysis
EvidenceLevel 1
PublishedSep 2026
View Original Abstract ↓
BackgroundPhospholipids are important bioactive components in human milk. They are rich in unsaturated fatty acids and play crucial roles in cell signal transduction and infant intellectual development.MethodsThis study quantitatively pooled concentrations of total phospholipids and their subclasses in human milk, and narratively summarized phospholipid subclass proportions across different countries. Relevant studies published up to October 2025 were searched from six databases. After systematic search, a total of 25 studies were finally included, among which 18 studies on phospholipid concentrations were subjected to meta-analysis.ResultsResults from the random-effects model showed that the total phospholipid concentration was 251.60 mg/L (175.62, 327.58) in colostrum, 234.71 mg/L (171.07, 298.35) in transitional milk, 197.00 mg/L (166.08, 227.93) in mature milk. Throughout lactation, PC, SM, and PE are the major phospholipid classes in human milk. Subgroup meta-analyses indicated that detection methods were a significant effect for total phospholipids, PE, and PI concentrations within mature milk.ConclusionThis systematic review provides updated estimates of phospholipids and their subclasses concentrations in term human milk, and provides a theoretical basis for the application of phospholipids in infant formula.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, Identiffer CRD420261445346.
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