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Traditional Chinese medicine shows heterogeneous effects on airway and gut microbial abundance in animal modelsTraditional Chinese Medicine Shows Mixed Effects on Gut and Airway Bacteria

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Key Takeaway
Note that traditional Chinese medicine shows inconsistent effects on airway and gut microbial diversity in animal models.

This meta-analysis synthesized 20 studies using animal models to evaluate the effects of traditional Chinese medicine on microbial diversity indices and abundance in the context of chronic inflammatory airway disease. The analysis focused on gut and airway microbial relative abundance as primary and secondary outcomes.

The authors reported no statistically significant overall effect on gut and airway alpha-diversity indices. However, differences were reported in beta diversity between the model and traditional Chinese medicine groups. Results regarding the relative abundance of airway and gut microbes were noted as heterogeneous across the included studies.

Several limitations were identified, including substantial heterogeneity in pooled estimates and sensitivity to individual-study omission. The authors noted a high or unclear risk of bias in most SYRCLE domains. Because the evidence is derived solely from preclinical animal models, it does not provide evidence of microbiota restoration or specific therapeutic mechanisms. Clinical application is currently limited by the lack of human clinical trial data.

How this fits prior evidence

This meta-analysis addresses a gap in understanding how traditional Chinese medicine affects the microbiome in chronic inflammatory airway disease. While previous evidence suggests traditional Chinese medicine may modulate neuroimmunomodulatory pathways and immune balance in multiple sclerosis, this study specifically examines microbial diversity in airway disease models. The findings are limited to preclinical models and do not confirm clinical outcomes.

Researchers conducted a meta-analysis of 25 studies involving animal models to see how traditional Chinese medicine affects the microbial diversity in the gut and airways. These studies were specifically looking at conditions involving chronic inflammatory airway disease.

The results were mixed. While some differences were noted in the types of bacteria present between the groups, there was no significant overall effect on the diversity of the microbes. Additionally, the amount of specific bacteria found in the gut and airways varied greatly across the different studies included in the analysis.

It is important to note that this research was conducted on animals, not humans. Because the results were inconsistent and the study was small, these findings do not yet provide a clear picture of how these treatments might work in people. More human trials are needed to understand the safety and effectiveness of these treatments.

What this means for you:
Early animal studies show inconsistent results regarding how traditional Chinese medicine affects gut and airway bacteria.

Common questions

What did the study find about gut and airway bacteria?

The study found no statistically significant overall effect on the diversity of bacteria in the gut and airways. While some differences were reported in the types of bacteria between groups, the results were inconsistent across the different studies included in the analysis.

Was this study conducted on humans?

No, this was a preclinical study conducted using animal models. Because the research was performed on animals, the results cannot be used to determine how these treatments work for people with chronic inflammatory airway diseases.

Is traditional Chinese medicine proven to treat airway disease?

The current evidence is limited and does not prove that traditional Chinese medicine treats these conditions. The study showed inconsistent results and was conducted on animals, so more research is needed to understand its effects.

Study Details

Study typeMeta analysis
EvidenceLevel 1
PublishedSep 2026
View Original Abstract ↓
A growing number of studies have indicated that traditional Chinese medicine (TCM) can regulate microbiota in chronic inflammatory airway disease (CIAD). Nevertheless, no systematic review or integrated analysis has summarized relevant evidence to date, limiting the clinical application of TCM in this field. This study followed the PRISMA reporting guidelines. We systematically searched PubMed, EMBASE, the Cochrane Library and Web of Science for animal studies published from January 1, 2006 to January 5, 2026 exploring the effects of TCM on microbiota in CIAD. Microbial diversity indices and abundance data were extracted. Meta-analysis was performed using R version 4.5.0 with the meta for package (version 5.0.1), and risk of bias was assessed using the Systematic Review Centre for Laboratory Animal Experimentation (SYRCLE) tool. A total of 25 studies published from 2016 to 2025 were included, 20 of which were enrolled into the meta-analysis. No statistically significant overall effect of TCM on the examined gut or airway alpha-diversity indices was identified after re-analysis using REML random-effects models with Hartung–Knapp adjustment. Several pooled estimates showed substantial heterogeneity and sensitivity to individual-study omission. Most included studies reported differences in beta diversity between model and TCM groups. Changes in airway and gut microbial relative abundance were heterogeneous across studies. High or unclear risk of bias was identified in most SYRCLE domains for the included studies. Current preclinical evidence suggests that TCM may be associated with changes in airway and gut microbiota in CIAD animal models. However, the updated quantitative analyses did not demonstrate statistically significant overall effects on the alpha-diversity indices examined, and evidence concerning airway microbiota and microbial relative abundance remains heterogeneous. These findings should not be interpreted as evidence of microbiota restoration or therapeutic mechanisms. Well-designed studies with more standardized methodologies are warranted to clarify the impact of TCM on lung–gut axis microecology. https://www.crd.york.ac.uk/prospero/, identifier PROSPERO CRD420261385384.
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