Phase 2 N=5 Treatment

Effectiveness of the Investigational Drug Campath-1H in Preventing Rejection of Transplanted Kidneys

Graft Rejection · Kidney Disease

Bottom Line

View on ClinicalTrials.gov: NCT00001984 ↗

Enrolled (actual)

Serious AEs

100.0%

Results posted

Oct 2010

Primary outcome: Primary: Number of Patients With Renal Allograft Rejection — 5 participant

Study Design & Population

Study type: Interventional
Phase: Phase 2
Interventions: Alemtuzumab and DSG (Drug)
Age: Pediatric, Adult, Older Adult
Sex: All
Sponsor: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Primary completion: Jun 2008

Outcome Measures

Outcome	Result	p-value
PRIMARY Number of Patients With Renal Allograft Rejection	5	—
PRIMARY Rejection Day of Onset	28	—
PRIMARY Rise in Serum Creatineine Above Posttransplant Nadir	58	—
SECONDARY Creatinine Level at 6 Month Post Operation	1.5	—
SECONDARY Creatinine Level at Year 1 Post Operation	1.4	—
SECONDARY Creatinine at 2 Years	1.7	—
SECONDARY Monocyte Count	527	—

Summary

This protocol will test a humanized monoclonal antibody known as Campath-1H for its ability to induce a state of permanent allograft acceptance, or tolerance, when administered in combination with a brief course of the immunosuppressive drug deoxyspergualin (DSG) at the time of human renal allotransplantation. Campath-1H is specific for the common lymphocyte and monocyte antigen CD52. Its administration temporarily depletes mature lymphocytes and some monocytes without altering neutrophils or hematopoietic stem cells. Deoxyspergualin inhibits the NFkB pathway thus preventing monocyte and macrophage activation. Recipients of living or cadaveric donor kidneys will be treated with one dose of Campath-1H prior to transplantation to insure that peripheral depletion is achieved at the time of graft reperfusion. Three subsequent doses of Campath-1H will be administered on the first, third and fifth days after the transplant to deplete passenger donor leukocytes and residual recipient cells that mobilize in response to the allograft. In addition, patients will be treated with DSG for 14 days beginning on the day prior to surgery. This trial expands on pilot studies at the NIH of 15 patients in which Campath was given alone at the time of transplantation. In those studies, excellent peripheral depletion occurred after just one dose of Campath though central depletion required additional dosing. This allowed for greatly reduced immunosuppression to be used to prevent rejection, but to date, all patients have required some immunosuppressive medication. It is hoped that the addition of DSG will eliminate the need for long-term immunosuppression. Patients will be followed closely in the post transplant period. If patients experience rejection, they will be treated with methylprednisolone and have immunosuppression added using sirolimus as the predominant immunosuppressive agent. In the previous phase of this study without DSG, this maneuver has in all cases been successful in returning the allograft to normal function. In addition to evaluating graft function following transplantation, this protocol will also characterize and evaluate the function of the immune system and the composition of the T cell repertoire following the administration of Campath-1H and DSG, and during immune system recovery after transplantation.

Eligibility Criteria

INCLUSION CRITERIA:

Candidates for a kidney transplant performed at the Warren G. Magnuson Clinical Center.

Willingness and legal ability to give informed consent, or permission from a legal guardian.

Willingness to travel to the Clinical Center for protocol specific samples to be taken, or in some cases, the ability to send samples via overnight mail.

Availability of donor tissue for testing. This could include splenic or peripheral blood lymphocytes from a cadaveric donor or a willing living donor enrolled on the Clinic Center Living Donor Protocol who consents to periodic phlebotomy for peripheral blood lymphocyte isolation.

EXCLUSION CRITERIA

Immunosuppressive drug therapy at the time of or 2 months prior to enrollment. Specifically, candidates must not be taking prednisone, cyclosporine, tacrolimus, azathioprine, mycophenolate mofetil, antilymphocyte agents, cyclophosphamide, methotrexate, or other agents whose therapeutic effect is immunosuppressive.

Any condition that precludes serial follow-up.

Any active malignancy or any history of a hematogenous malignancy or lymphoma. Patients with primary, cutaneous basal cell or squamous cell cancers may be enrolled providing the lesions are appropriately treated prior to transplant.

Significant coagulopathy or requirement for anticoagulation therapy that would contraindicate protocol allograft biopsies.

Platelet count less than 100,000/mm(3).

Hemoglobin less than 9.0 mg/dl. Patients may be on erythropoietin therapy, but will not be placed on therapy solely to facilitate research sample acquisition.

Any known immunodeficiency syndrome.

HLA identical status with a living donor.

Any history of uncompensated cardiac insufficiency, major vascular disease, or symptomatic coronary artery disease.

Systemic or pulmonary edema.

Inability to be effectively dialyzed.

Chronic hypotension (SBP less than 100 mmHg).

Any condition that would likely increase the risk of protocol participation or confound the interpretation of the data.

CMV negative status receiving an organ from a known CMV positive donor.

EBV negative status receiving an organ from a known EBV positive donor.

Panel reactive antibody greater than 20% due to HLA antibodies.

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Data sourced from ClinicalTrials.gov (NCT00001984). Outcome figures and adverse-event rates are extracted automatically from the registry's posted results and are provided for clinician reference, not as a substitute for the primary publication.