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Phase 4 N=14 Treatment

Effects of PCSK9 Inhibition by Evolocumab on Postprandial Lipid Metabolism in Type 2 Diabetes

Type 2 Diabetes Mellitus

Enrolled (actual)
14
Serious AEs
23.1%
Results posted
Oct 2021
Primary outcome: Primary: Mean ApoB Concentration Before and After Evolocumab — 75.9; 35.3 mg/dL — p=<0.001

Study Design & Population

Study type
Interventional
Phase
Phase 4
Interventions
Evolocumab (Drug)
Age
Adult, Older Adult · 18+ yrs
Sex
All
Sponsor
Marja-Riitta Taskinen
Primary completion
Jun 2018

Outcome Measures

OutcomeResultp-value
PRIMARY
Mean ApoB Concentration Before and After Evolocumab
75.9; 35.3 <0.001 sig
PRIMARY
Mean TRL-C Concentration Before and After Evolocumab
33.4; 17.8 <0.001 sig
PRIMARY
Mean Total Production of ApoB48 Before and After Evolocumab
570; 580 0.31
PRIMARY
Mean LDL FCR of ApoB100 Before and After Evolocumab
0.32; 0.78 <0.001 sig
PRIMARY
LDL Pool Size of ApoB100 Before and After Evolocumab
1500; 460 <0.001 sig
SECONDARY
Mean LDL-C Concentration Before and After Evolocumab
1500; 460 <0.001 sig
SECONDARY
Mean ApoB48 Concentration Before and After Evolocumab
6.1; 5.3 0.27
SECONDARY
Mean CM TG-iAUC Before and After Evolocumab
6.8; 5.8 0.22
SECONDARY
Mean ApoB48 AUC Before and After Evolocumab
66.8; 57.4 0.31
SECONDARY
Mean VLDL1 ApoB100 Production Before and After Evolocumab
790; 750 0.61
SECONDARY
Mean VLDL2 ApoB100 Production Before and After Evolocumab
270; 230 0.11
SECONDARY
IDL Pool Size of ApoB100 Before and After Evolocumab
190; 130 0.003 sig
SECONDARY
Mean IDL to LDL Transfer of ApoB100 Before and After Evolocumab
450; 310 0.027 sig
SECONDARY
Mean VAT Before and After Evolocumab
2420; 2450 0.84
SECONDARY
Mean Liver Fat Before and After Evolocumab
6.6; 6.3 0.59
SECONDARY
Mean SAT Before and After Evolocumab
3900; 4110 0.81
SECONDARY
Mean VLDL1 Triglyceride Production Before and After Evolocumab
34; 33 0.91
SECONDARY
Mean VLDL1 FCR of triglycerideBefore and After Evolocumab
38; 37 1
SECONDARY
Mean VLDL2 Triglyceride Total Production Before and After Evolocumab
8.8; 8.5 0.68
SECONDARY
Mean VLDL2 FCR of Triglyceride Before and After Evolocumab
10; 15 0.013 sig
SECONDARY
Mean Postprandial CM of ApoB48 Before and After Evolocumab
240; 230 0.91
SECONDARY
Mean CM-apoB48 FCR of ApoB48 Metabolism Before and After Evolocumab
37; 46 0.27
SECONDARY
Mean CM-TG Production of ApoB48 Before and After Evolocumab
66.5; 66.5
SECONDARY
Mean CM TG-AUC Before and After Evolocumab
9.5; 8.9 0.84
SECONDARY
Mean ApoB48 iAUC Before and After Evolocumab
18.4; 13.9 0.89

Summary

Postprandial lipemia is highly prevalent in type 2 diabetes subjects even with normal fasting triglyceride values. Humans are mostly in a postprandial rather than in a fasting state and therefore non-fasting triglyceride values reflect more accurately the continuous exposure of arterial wall to the substantial cholesterol load from remnant particles. Evolocumab lowers blood LDL-cholesterol. This study evaluates the effect of evolocumab on postprandial lipid metabolism in type 2 diabetes. All participants in this study receive evolocumab treatment.

Eligibility Criteria

Inclusion Criteria

  • Male or female (non-fertile or using a medically approved birth control method) overweight/obese subjects with Type 2 Diabetes Mellitus treated with lifestyle counselling and a stable metformin dose for at least three months
  • age 18-77 yrs.
  • body mass index 25-40 kg/m2
  • triglycerides between 1.5-4.5 mmol/L and low-density-lipoprotein cholesterol >1.8 but ≤4.0 mmol/L (on Atorvastatin 20 mg/day)
  • glycohemoglobin: ≤9%.
  • Each patient will attend a pre-screening visit (at week -5) where eligibility criteria will be evaluated. If the patient uses another statin than atorvastatin (20 mg) at screening visit the used statin is stopped and atorvastatin 20 mg will be initiated. If the patient is not using any statin, atorvastatin 20 mg will be initiated and the lipid values will be checked after 4 weeks when all inclusion/exclusion criteria will be assessed.

Exclusion Criteria

  • Type 1 diabetes
  • apolipoprotein E2/2 phenotype
  • alanine transaminase / aspartate transaminase > 3× upper limit of normal
  • creatinine kinase>3× upper limit of normal
  • glomerular filtration rate 40 kg/m2
  • glycohemoglobin > 9.0 %
  • fasting triglycerides > 4.5 mmol/l
  • total cholesterol > 7.0 mmol/l
  • positive urine or serum pregnancy test
  • untreated or inadequately treated hypertension defined as blood pressure >160 mmHg systolic and/or >105 mmHg diastolic, use of thiazide diuretics at a dose of ≥25 mg/day
  • subject not on a stable dose of atorvastatin (20 mg/ day before randomization)
  • lipid-lowering drugs other than statins within 3 months
  • any other diabetes medication except diet + metformin
  • history/diagnosis of diabetes nephropathy / retinopathy
  • current smoking
  • weekly alcohol use over 24 doses for men and 16 for women
  • history of myocardial infarction, acute coronary syndrome or coronary revascularization (percutaneous coronary intervention or coronary artery bypass grafting) within the last 6 mos.
  • planned revascularization (eg coronary artery bypass grafting, percutaneous coronary intervention, carotid or peripheral revascularization procedures) within 3 months of screening
  • New York Heart Association class III/IV congestive heart failure persisting despite treatment
  • history of hemorrhagic stroke
  • hypersensitivity to (evolocumab or) any of the excipients found in the drug product
  • use of estrogen therapy
  • current use of antithrombotic or anticoagulant therapy
  • known bleeding tendency that would be an contraindication to heparin test
  • history of cancer within the past 5 years (except for adequately treated basal cell skin cancer, squamous cell skin cancer or in situ cervical cancer)
  • women of childbearing potential not protected by effective birth control method and/or not willing to be tested for pregnancy
  • patient considered by the investigator or any sub-investigator as inappropriate for this study for any reason
View full record on ClinicalTrials.gov →

Data sourced from ClinicalTrials.gov (NCT02948777). Outcome figures and adverse-event rates are extracted automatically from the registry's posted results and are provided for clinician reference, not as a substitute for the primary publication.

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