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N/A N=20 Randomized Double-blind Basic Science

Essential Amino Acids and Protein Kinetics During Caloric Deprivation

Muscle Protein Synthesis · Whole-body Protein Balance

Enrolled (actual)
20
Serious AEs
0.0%
Results posted
Nov 2020
Primary outcome: Primary: Postprandial, Resting Muscle Protein Synthesis Rates — 0.055; 0.061 percent per hour

Study Design & Population

Study type
Interventional
Phase
N/A
Interventions
Standard EAA (Dietary_supplement); High EAA (Dietary_supplement)
Age
Adult · 18+ yrs
Sex
All
Sponsor
United States Army Research Institute of Environmental Medicine
Primary completion
Mar 2019

Outcome Measures

OutcomeResultp-value
PRIMARY
Postprandial, Resting Muscle Protein Synthesis Rates
0.055; 0.061
PRIMARY
Postprandial, Post-exercise Muscle Protein Synthesis Rates
0.055; 0.065
PRIMARY
How Well Participants Suppress the Degradation of Body Proteins While Stimulating the Growth of New Proteins After Ingesting Varying Doses of EAA at Rest and After Exercise. Net Whole-body Protein Balance
2.7; 21.3

Summary

The amount of essential amino acids (EAA) necessary to maximally stimulate muscle protein synthesis and optimize whole-body net protein balance during caloric deprivation has not been determined. This study will address that gap in knowledge by examining the resting and post-exercise muscle and whole-body protein kinetic responses to ingesting varying amounts of EAA after a 5 day period of negative energy balance. This study will provide the initial evidence to support the development of a recovery-based food product for military combat rations.

Eligibility Criteria

Inclusion Criteria

  • Men and women aged 18 - 35 years
  • Body mass index < 30.0 kg/m2
  • Healthy without evidence of chronic illness or musculoskeletal injury as determined by the USARIEM Office of Medical Support and Oversight (OMSO)
  • Resistance exercise trained defined by self-report as performing ≥ 2 sessions/wk for previous 6 mo
  • Refrain from taking any nonsteroidal anti-inflammatory drugs (e.g., aspirin, Advil®, Aleve®, Naprosyn®), or any other aspirin-containing product for 10 days before starting and at least 5 days after completing the study
  • Willing to refrain from alcohol, smoking any nicotine product (includes e-cigarettes); vaping, chewing tobacco, caffeine, and dietary supplement use throughout the entire study period
  • Supervisor approval for federal civilian employees and non-HRV active duty military personnel working within the US Army Natick Soldier Systems Center

Exclusion Criteria

  • Musculoskeletal injuries that compromise exercise capability as determined by the USARIEM Office of Medical Support and Oversight (OMSO)
  • Metabolic or cardiovascular abnormalities, gastrointestinal disorders (e.g., kidney disease, diabetes, cardiovascular disease, etc.)
  • Abnormal PT/PTT test or problems with blood clotting
  • History of complications with lidocaine
  • Present condition of alcoholism, anabolic steroids, or other substance abuse issues
  • Blood donation within 8-wk of beginning the study
  • Pregnancy (self-report or results of urine pregnancy test before body composition testing)
  • Unwillingness or inability to consume study diets or foods provided
View full record on ClinicalTrials.gov →

Data sourced from ClinicalTrials.gov (NCT03372928). Outcome figures and adverse-event rates are extracted automatically from the registry's posted results and are provided for clinician reference, not as a substitute for the primary publication.

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