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Phase 1 N=29 Randomized Quadruple-blind Basic Science

Development of Pregnenolone as a Treatment for Depression

Major Depressive Disorder

Enrolled (actual)
29
Serious AEs
0.0%
Results posted
May 2024
Primary outcome: Primary: Amygdala-PCC Functional Connectivity — -0.0815; -0.0530; -0.0456 z-score — p=.195

Study Design & Population

Study type
Interventional
Phase
Phase 1
Interventions
Pregnenolone 500 mg (Drug); Pregnenolone 800 mg (Drug); Placebo (Drug)
Age
Adult, Older Adult · 18+ yrs
Sex
Female
Sponsor
University of Texas Southwestern Medical Center
Primary completion
May 2022

Outcome Measures

OutcomeResultp-value
PRIMARY
Amygdala-PCC Functional Connectivity
-0.0815; -0.0530; -0.0456 .195
PRIMARY
dlPFC-Insula Functional Connectivity
0.0495; 0.1413; 0.0855 .468
PRIMARY
GABA Concentration.
0.0947; 0.0861; 0.0974 .316
SECONDARY
Pregnenolone Level
10902.60; 11484.75; 769.63 .006 sig
SECONDARY
Allopregnanolone Level
2478.39; 2489.08; 124.22 .001 sig
SECONDARY
Systematic Assessment for Treatment Emergent Events (SAFTEE)
16.15; 19.14; 17.60 .336
SECONDARY
Pregnenolone Dose
500

Summary

Pregnenolone, an over-the-counter supplement, is a naturally occurring neurosteroid made in the adrenal glands and brain. Preclinical research suggests pregnenolone has antidepressant, cognitive enhancing, and neuroprotective properties, particularly in women. The following hypothesis will be tested in this trial: pregnenolone is associated with improvement in depressive symptom severity in women that is associated with changes in the resting state functional connectivity (rsFC) and GABA.

Eligibility Criteria

Inclusion Criteria

  • Women, ages 18-65 years, with current MDD (mild or moderate severity per DSM-5) based on SCID-CV.
  • No psychotropic medications, other than PRN (as needed) hypnotics, within 28 days of randomization (medication free).
  • PRN hypnotics allowed up to 3 days prior to study drug administrations but not while receiving study drug.

Exclusion Criteria

  • Severe MDD based on DSM-5 severity criteria and/or a baseline HRSD score > 27 (consistent with severe depressive symptom severity).
  • High risk for suicide (active SI with plan/intent or > 2 lifetime attempts in lifetime or any in the past 6 months).
  • Treatment resistant depression (fail two adequate antidepressant trials or ECT during current episode).
  • Vulnerable populations (e.g. pregnant/nursing, severe cognitive or intellectual impairment, incarcerated).
  • Coronary artery disease, atrial fibrillation, stroke, deep vein thrombosis, pulmonary embolism or blood clotting disorder, or any severe, life threatening or unstable, medical condition.
  • History of allergic reaction or side effects with prior pregnenolone use.
  • Current substance use disorder defined as meeting criteria for a use disorder based on the SCID interview and self-reported use within the past 3 months, or a positive baseline urine drug screen.
  • Current psychotic features (hallucinations, delusions, disorganized thought processes) or eating disorders.
  • Anxiety disorders of sufficient severity to be the primary focus of clinical attention (e.g. severe obsessive compulsive or post-traumatic stress disorders).
  • Hormone-sensitive conditions (i.e. breast cancer; uterine/ovarian cancer, endometriosis, uterine fibroids).
  • Clinically significant laboratory, physical examination, or electrocardiogram (ECG) findings.
  • Currently using oral contraceptives containing progestin (barrier methods allowed).
View full record on ClinicalTrials.gov →

Data sourced from ClinicalTrials.gov (NCT03645096). Outcome figures and adverse-event rates are extracted automatically from the registry's posted results and are provided for clinician reference, not as a substitute for the primary publication.

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