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Phase 3 Completed N=698 Treatment

Microbiota Restoration Therapy for Recurrent Clostridium Difficile Infection (PUNCH CD3-OLS)

Clostridium Difficile Infection · Infections · Communicable Diseases
Source: ClinicalTrials.gov NCT03931941 ↗
Enrolled (actual)
698
Serious AEs
12.5%
Results posted
Aug 2024
Primary outcomePrimary: Safety and Tolerability of RBX2660 in Subjects With Recurrent CDI. — 697 Participants
◆ Published Evidence
Established
32citations · ~32 / year
PUNCH CD3-OLS: A Phase 3 Prospective Observational Cohort Study to Evaluate the Safety and Efficacy of Fecal Microbiota, Live-jslm (REBYOTA) in Adults With Recurrent Clostridioides difficile Infection.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2025 · Open access · Likely link

Summary

This is a prospective, multicenter, open-label Phase 3 study of a microbiota suspension of intestinal microbes. Patients who have had at least one recurrence of CDI after a primary episode and have completed at least one round of standard-of-care oral antibiotic therapy or have had at least two episodes of severe CDI resulting in hospitalization may be eligible for the study. Subjects may receive a second RBX2660 enema if they are deemed treatment failures following the initial enema per the protocol-specified treatment failure definition.

Linked Publications (5)

  • PUNCH CD3-OLS: A Phase 3 Prospective Observational Cohort Study to Evaluate the Safety and Efficacy of Fecal Microbiota, Live-jslm (REBYOTA) in Adults With Recurrent Clostridioides difficile Infection.
    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2025 · 32 citations · Open access · Likely link
  • Safety and Efficacy of Fecal Microbiota, Live-jslm, in Preventing Recurrent <i>Clostridioides difficile</i> Infection in Participants Who Were Mildly to Moderately Immunocompromised in the Phase 3 PUNCH CD3-OLS Study.
    Open Forum Infectious Diseases · 2025 · 7 citations · Open access · Likely link
  • Safety and Efficacy of Fecal Microbiota, Live-jslm to Prevent Recurrent Clostridioides difficile Infection in Participants With Irritable Bowel Syndrome.
    Infectious diseases and therapy · 2025 · 3 citations · Open access · Likely link
  • Integrated analysis of the safety of fecal microbiota, live-jslm in adults with recurrent <i>Clostridioides difficile</i> infection from five prospective clinical trials: an update.
    Therapeutic advances in gastroenterology · 2025 · 1 citation · Open access · Likely link
  • Safety and Efficacy of Fecal Microbiota, Live-jslm for Prevention of Recurrent Clostridioides difficile Infection Among Hospitalized Participants in PUNCH CD3-OLS.
    Infectious diseases and therapy · 2026 · 0 citations · Open access · Likely link

Outcome Measures

OutcomeResultp-value
PRIMARY
Safety and Tolerability of RBX2660 in Subjects With Recurrent CDI.
697
SECONDARY
Efficacy of RBX2660 Measured at 8 Weeks After Treatment.
499
SECONDARY
Sustained Clinical Response Through 6 Months After Treatment.
454

Eligibility Criteria

Inclusion Criteria

  • ≥ 18 years old.
  • Medical record documentation of either: a) a current diagnosis or history of recurrent CDI as determined by the treating physician, b) or has had at least two episodes of severe CDI resulting in hospitalization.
  • Is currently taking or was just prescribed antibiotics to control CDI related diarrhea at the time of enrollment. [Note: Subject's CDI diarrhea must be controlled (<3 unformed/loose stools/day) while taking antibiotics during screening.]

Exclusion Criteria

  • Has continued CDI diarrhea despite being on a course of antibiotics prescribed for CDI treatment.
  • Requires systemic antibiotic therapy for a condition other than CDI.
  • Fecal microbiota transplant (FMT) within the past 6 months.
  • FMT with an associated serious adverse event related to the FMT product or procedure.
  • Bezlotoxumab (CDI monoclonal antibodies) if received within the last year.
  • CD4 count <200/mm^3 during Screening.
  • An absolute neutrophil count of <1000 cells/µL during Screening.
  • Pregnant, breastfeeding, or intends to become pregnant during study participation.
View full record on ClinicalTrials.gov →

Data sourced from ClinicalTrials.gov (NCT03931941) and the linked publication. Outcome figures and adverse-event rates are extracted automatically from the registry's posted results and are provided for clinician reference, not as a substitute for the primary publication. Informational only — not medical advice.

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