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N/A N=42

Clinical Investigation Study to Evaluate the Consistency and Reproducibility of Two Consecutive Mosquito Feeding Assays

Malaria

Enrolled (actual)
42
Serious AEs
0.0%
Results posted
Apr 2024
Primary outcome: Primary: Oocyst Prevalence — 5.2; 6.3; 2.3; 2.2 percentage of mosquitoes — p=0.711

Study Design & Population

Study type
Observational
Phase
N/A
Interventions
Direct Skin Feeding Assay (DSFA) (Other); Primaquine (Drug); Artemether/Lumefantrine (Drug)
Age
Adult · 18+ yrs
Sex
All
Sponsor
PATH
Primary completion
Dec 2021

Outcome Measures

OutcomeResultp-value
PRIMARY
Oocyst Prevalence
5.2; 6.3; 2.3; 2.2 0.711
SECONDARY
Oocyst Density
1.1; 1.6; 0.4; 0.4 0.019 sig
SECONDARY
Sporozoite Prevalence
5.1; 4.5; 1.8; 2.0 0.750
SECONDARY
Sporozoite Density

Summary

The proposed trial design has been developed to assess the consistency and reproducibility of two consecutive direct skin feeding assays (DSFA) at 24-hour interval.

Eligibility Criteria

Inclusion Criteria

  • Provision of signed or thumb printed and dated informed consent form
  • Stated willingness to comply with all study procedures and availability for the duration of the study
  • Male or female aged between 18 years and 55 years inclusive.
  • Resident within the study area
  • In good general health as evidenced by medical history and clinical examination before entering the study Ability to take oral Coartem and low-dose primaquine anti-malarials upon conclusion of day 2 (2nd direct skin feed) and be willing to adhere to the medication regimen
  • For females, she must be of non-childbearing potential or use appropriate measures to prevent pregnancy for 30 days after receiving Coartem and primaquine. Non-childbearing potential means she is surgically sterilized or at least one year post-menopausal. Appropriate measures to prevent pregnancy include abstinence or adequate contraceptive precautions (i.e. intrauterine contraceptive device; oral contraceptives; diaphragm or condom in combination with contraceptive jelly, cream or foam; Norplant or Depo-Provera).
  • For males, he must be willing to ensure that he does not get his partner(s) pregnant for at least 3 months after treatment with primaquine. Appropriate measures to prevent pregnancy include abstinence or adequate contraceptive precautions in either the participant or the partner.
  • Positive for P. falciparum gametocytes as measured by polymerase chain reaction (PCR) with cycle threshold (cT) value < 31.

Exclusion Criteria

Presence of any signs or symptoms of malaria

  • Presence of contraindications to administration of Coartem and primaquine as indicated in the respective drug package inserts
  • History of severe allergic reactions to mosquito bites (other than pruritus and local swelling)
  • Pregnant (i.e. a positive pregnancy test)
  • Current or recent (within the preceding 2 weeks) use of antimalarial treatment
  • Current participation in a malaria vaccine study
  • Any other findings that the investigator feels would increase the risk of having an adverse outcome from participation in the trial.
View full record on ClinicalTrials.gov →

Data sourced from ClinicalTrials.gov (NCT04666350). Outcome figures and adverse-event rates are extracted automatically from the registry's posted results and are provided for clinician reference, not as a substitute for the primary publication.

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