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N/A N=5 Basic Science

Sympathetic Activation in Obesity

Obesity · Healthy

Enrolled (actual)
5
Serious AEs
0.0%
Results posted
Nov 2023
Primary outcome: Primary: Distribution Volume Ratio (DVR) of [11C]MRB PET Imaging of Peripheral Sympathetic Nervous System — 0.13; -0.025; 0.095; -0.03 DVR

Study Design & Population

Study type
Interventional
Phase
N/A
Interventions
PET Scan (Diagnostic_test); Mixed Meal Test (Dietary_supplement); Muscle sympathetic nerve activity (MSNA) (Diagnostic_test); Oral Glucose Tolerance Test (Diagnostic_test); Body Composition, Percent Body Fat & Percent Body Water (Tanita scale) (Other); CT Scan (Diagnostic_test); Body Composition, Percent Body Fat & Percent Body Water (DXA Scan) (Other)
Age
Adult · 18+ yrs
Sex
All
Sponsor
Yale University
Primary completion
Sep 2022

Outcome Measures

OutcomeResultp-value
PRIMARY
Distribution Volume Ratio (DVR) of [11C]MRB PET Imaging of Peripheral Sympathetic Nervous System
0.13; -0.025; 0.095; -0.03; -0.095; -0.195
PRIMARY
Microneurography (Muscle Sympathetic Nerve Activity (MSNA)) Measured by Burst Frequency
11.18; 9.541; 15.63; 11.60
SECONDARY
DVR of Norepinephrine Transporter (NET) in the Central Nervous System (CNS)
0.549; 0.525
SECONDARY
DVR of [11C]MRB PET Imaging of Peripheral Sympathetic Nervous System After Mixed Meal Test
0.577; 0.491; 0.521; 0.559
SECONDARY
Plasma Glucose
SECONDARY
Insulin Levels
7.07; 15.55; 61.06; 78.37
SECONDARY
C-Peptide
SECONDARY
Plasma Catecholamines (Epinephrine and Norepinephrine)
0.0487; 0.0131; 0.0155; 0.0136; 0.146; 0.135
SECONDARY
Plasma Free Fatty Acids (FFA)
380; 412; 419; 404
SECONDARY
Thyroid Function Tests
SECONDARY
Electrocardiogram (EKG)- Heart Rate
61.8; 74.2; 65.6; 71.7
SECONDARY
Electrocardiogram (EKG) - RR Interval
0.94; 1.01; 0.91; 0.85

Summary

The primary objective of this study is to determine whether sympathetic nervous system (SNS) activity in white adipose tissue (WAT) and brown adipose tissue (BAT), skeletal muscle and brain is altered in individuals with obesity in comparison to individuals with normal weight. Simultaneous multi-organ SNS activation will be obtained with a radiotracer for norepinephrine transporter (NET) for whole-body Positron Emission Tomography (PET) imaging and microneurography (peroneal muscle SNS activity). The secondary objectives of this study are: 1) to evaluate whether gender differences affect peripheral SNS in healthy normal weight and obese men and women in adipose tissue (WAT and BAT) and muscle (resting/fasting); 2) To investigate the relationship between peripheral and central SNS activity in obesity, by correlating SNS activity in peripheral tissues (WAT, BAT, and muscle) and brain; and 3) To investigate NET CNS and peripheral SNS activity before and after a high carbohydrate mixed meal in lean and obese men and women.

Eligibility Criteria

Inclusion Criteria:In order to be eligible to participate in this study, an individual must meet all of the following criteria:

  • Provision of signed and dated informed consent form
  • Stated willingness to comply with all study procedures and availability for the duration of the study
  • Age 18-45 years
  • In good general health as evidenced by medical history and lean (BMI 18.5-24.9 kg/m2) or non-diabetic obese (BMI 30-50 kg/m2) with a fasting plasma glucose (FPG) <100 mg/dL and a hemoglobin A1c <5.7%.

Exclusion Criteria

An individual who meets any of the following criteria will be excluded from participation in this study:

  • Hypertension
  • Cardiac or pulmonary disease,
  • Known history of Type 1 or Type 2 diabetes
  • Hepatic disease, swallowing and gastrointestinal disorders
  • Current use of anti-obesity medications, supplements and/or anti-hyperglycemic medications
  • Neurological injury or illness, and psychiatric medications
  • Women who are pregnant or lactating
  • Subjects who suffer from claustrophobia
  • Subjects who have received a diagnostic or therapeutic radiopharmaceutical within 7 days prior to participation in this study
  • Radiation works or participation in other research studies involving ionizing radiation within one year of the PET scans that would cause the subject to exceed the yearly dose limits.
  • Subjects with history of IV drug use which would prevent venous access for PET tracer injection
  • Severe motor problems that prevent the subject from lying still for PET and MR imaging
  • Subjects who complain of chronic pain
  • Blood donation within 8 weeks of the study
View full record on ClinicalTrials.gov →

Data sourced from ClinicalTrials.gov (NCT04946552). Outcome figures and adverse-event rates are extracted automatically from the registry's posted results and are provided for clinician reference, not as a substitute for the primary publication.

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