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N/A Completed N=8 Basic Science

Monocyte Priming When Consuming a Western Diet

Source: ClinicalTrials.gov NCT05348395 ↗
Enrolled (actual)
8
Serious AEs
0.0%
Results posted
Mar 2026
Primary outcomePrimary: Measurement of Mitogen-activated Protein Kinase Phosphate 1 (MKP-1) Activity — 6.71 ng/mg protein

Summary

To determine the mechanism of monocyte priming in humans, the study team will conduct a complete feeding trial in normal weight and metabolically healthy human subjects (20-45 years of age) using a western diet (WD), characterized as being high-saturated fat, high-fructose, and high-calorie for 8 weeks.

Outcome Measures

OutcomeResultp-value
PRIMARY
Measurement of Mitogen-activated Protein Kinase Phosphate 1 (MKP-1) Activity
5.91
PRIMARY
Measurement of Mitogen-activated Protein Kinase Phosphate 1 (MKP-1) Activity
5.91
SECONDARY
Ribonucleic Acid (RNA) Analysis

Eligibility Criteria

Inclusion Criteria

  • Age 20-45
  • Planning to be available for the entire study period
  • Able to speak and read English
  • Normal weight (body mass index 18.5-24.9 kg/m2)
  • Able to eat the prescribed diet
  • Non-smoker

Exclusion Criteria

  • Excessive alcohol consumption
  • History of chronic cardiometabolic disease or major risk factor, including diabetes, hypertension, hyperlipidemia, heart disease
  • History of prior surgical procedure for weight control or liposuction
  • Use of weight loss medications in previous 6 months
  • Recent self-reported weight change
  • Severe pulmonary disease requiring supplemental oxygen
  • Abnormal renal or liver function
  • History of non-skin cancer in the past 5 years
  • Regular use of medications (prescribed or over-the-counter) that affect blood pressure, lipids, glucose, inflammation, or body weight
  • Works night shifts
  • Exercise per week > 420 minutes total for moderate activity or > 210 minutes for vigorous activity
  • Any medical or behavioral indication that would make participation unsafe based on the judgement of the study physician
  • Pregnant or lactating women
  • Known or discovered intolerances, allergies or difficulty consuming any of the foods included in the study diets
View full record on ClinicalTrials.gov →

Data sourced from ClinicalTrials.gov (NCT05348395). Outcome figures and adverse-event rates are extracted automatically from the registry's posted results and are provided for clinician reference, not as a substitute for the primary publication. Informational only — not medical advice.

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