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Systematic review and meta-analysis of DDS-based chemotherapy in murine colon cancer modelsTargeted drug delivery shrinks colon cancer tumors in mice by over half

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Key Takeaway
Consider DDS platforms for colon cancer based on preclinical murine efficacy data.

This systematic review and meta-analysis examines the efficacy of DDS-based chemotherapy in murine models of colon cancer. The authors synthesized data from 539 animals involved in various in vivo animal studies. The scope includes comparisons against controls, free-drug administration, and different delivery methods. The study is classified as a systematic review and meta-analysis rather than a primary clinical trial.

Tumor growth inhibition was significantly reduced when comparing DDS-based chemotherapy to controls. The weighted mean difference was -557.7 with a 95% CI of -716.8 to -398.5 and a p-value less than 0.001. When compared to free-drug administration, tumor growth was also significantly reduced with a weighted mean difference of -276.3 and a 95% CI of -367.6 to -185.1; p < 0.001.

Targeted DDS demonstrated significantly greater efficacy than non-targeted DDS. The weighted mean difference was -240.3 with a 95% CI of -399.4 to -81.25 and a p-value of 0.003. Intravenous administration showed significantly greater efficacy than intraperitoneal delivery, though specific effect sizes and confidence intervals were not reported for this comparison.

Safety data such as adverse events, serious adverse events, discontinuations, and tolerability were not reported. The authors note moderate methodological quality and variability in reporting of randomization, blinding, and sample size calculations. These preclinical findings do not imply clinical efficacy. The results underscore the translational potential of DDS platforms for rational design.

Imagine a treatment that hits cancer cells harder while sparing healthy tissue. That is exactly what a large review of animal studies suggests might be possible. Researchers looked at fifty-three-nine mice with colon cancer to see how different drug delivery methods worked. The results were clear and promising for future human trials.

When scientists used a specific drug called SN-38 wrapped in a targeted delivery system, tumor growth dropped significantly. This approach worked better than giving the drug freely without targeting or using a different drug called doxorubicin. The targeted method reduced tumor growth by a much larger amount than the non-targeted version.

Even the way the drug entered the body mattered. Giving the drug through a vein worked better than injecting it into the belly cavity. The review noted that the studies had some limitations in how they were designed. However, the overall picture points to a smarter way to fight cancer in the lab.

What this means for you:
Targeted delivery systems shrink colon cancer tumors more effectively in mice than standard methods.

Study Details

Study typeMeta analysis
EvidenceLevel 1
PublishedMay 2026
View Original Abstract ↓
BACKGROUND: Drug delivery systems (DDS) offer a promising strategy to enhance the therapeutic index of chemotherapeutic agents in colon cancer by improving tumor targeting, circulation time, and controlled drug release. Despite extensive preclinical research, a quantitative synthesis evaluating the efficacy of DDS and the influence of design parameters remains lacking. METHODS: We conducted a systematic review and meta-analysis of preclinical in vivo studies assessing DDS-based chemotherapy in murine models of colon cancer. A comprehensive search of PubMed, EMBASE, Scopus, Google Scholar, Cochrane CENTRAL, and ClinicalTrials.gov was performed through October 15, 2025. Outcomes included tumor growth inhibition, with subgroup analyses examining the effects of DDS platform, chemotherapeutic agent, targeting strategy, ligand type, and route of administration. RESULTS: Twenty-three studies comprising 25 experiments and 539 animals were included. Overall, DDS-based therapies significantly reduced tumor growth compared with controls ((WMD: - 557.7; 95% CI: - 716.8 to - 398.5; I²=88%; p < 0.001) and free-drug administration ((WMD: - 276.3; 95% CI: - 367.6 to - 185.1; I²=78%; p < 0.001). Both targeted and non-targeted DDS significantly reduced tumor growth compared with free drug treatment, while targeted DDS showed significantly greater efficacy than non-targeted systems (WMD: - 240.3; 95% CI: - 399.4 to - 81.25; I²=59%; p = 0.003). Although no statistically significant differences were observed between DDS platforms or chemotherapeutic agent subgroups, micelle-based systems and DDS formulations incorporating SN-38 or doxorubicin tended to show greater tumor growth reduction. Intravenous administration demonstrated significantly greater efficacy than intraperitoneal delivery, while hyaluronic acid- and aptamer-based targeting strategies achieved the largest tumor growth inhibition. Risk-of-bias assessment indicated moderate methodological quality, with variability in reporting of randomization, blinding, and sample size calculations. CONCLUSIONS: DDS-based chemotherapy consistently improves antitumor efficacy in preclinical colon cancer models, with targeting strategy, platform type, chemotherapeutic agent, and administration route influencing outcomes. These findings support the rational design of DDS platforms and underscore their translational potential. Rigorous preclinical study design and standardized reporting of efficacy and safety are essential to facilitate clinical translation.
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