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Phase 2 N=24 Randomized Triple-blind Treatment

Treatment of Degenerative Disc Disease With Allogenic Mesenchymal Stem Cells (MSV)

Degenerative Disc Disease · Intervertebral Disc Disease · Low Back Pain

Enrolled (actual)
24
Serious AEs
4.2%
Results posted
Apr 2024
Primary outcome: Primary: Pain and Disability Evaluation — 0.28; 0.15 scores from 0 to 1

Study Design & Population

Study type
Interventional
Phase
Phase 2
Interventions
Allogenic Mesenchymal Stromal Cells (Biological); Mepivacaine (Drug)
Age
Adult, Older Adult · 18+ yrs
Sex
All
Sponsor
Red de Terapia Celular
Primary completion
Dec 2015

Outcome Measures

OutcomeResultp-value
PRIMARY
Pain and Disability Evaluation
0.28; 0.15
SECONDARY
Evaluation of Affected Disc(s) by Quantitative MRI Ratio 12/6months
0.22; 0.06
SECONDARY
Evaluation of Affected Disc(s) by Quantitative Magnetic Resonance Imaging (RMI): Density at 6 Months
0.42; 0.51
SECONDARY
Evaluation of Affected Disc(s) by Quantitative Magnetic Resonance Imaging (RMI): Density at 12 Months
0.52; 0.46
SECONDARY
Visual Analogue Scale at 3 Months
43; 46
SECONDARY
Visual Analogue Scale at 6 Months
40; 51
SECONDARY
Visual Analogue Scale at 12 Months
47; 47
SECONDARY
Oswestry Disability Index at 3 Months
16; 25
SECONDARY
Oswestry Disability Index at 6 Months
20; 30
SECONDARY
Oswestry Disability Index at 12 Months
22; 34
SECONDARY
SF-12 Physical Component at 3 Months
47; 43
SECONDARY
SF-12 Physical Component at 6 Months
46; 39
SECONDARY
SF-12 Physical Component at 12 Months
45; 42
SECONDARY
SF-12 Mental Component at 3 Months
50; 46
SECONDARY
SF-12 Mental Component at 6 Months
52; 48
SECONDARY
SF-12 Mental Component at 12 Months
48; 50
SECONDARY
Pfirrmann Stage at 6 Months
0; 1; 10; 8; 2; 3
SECONDARY
Pfirrmann Stage at 12 Months
1; 1; 9; 4; 1; 7

Summary

In this study we want to evaluate the clinical use of allogenic mesenchymal stem cells (MSC), obtained from bone marrow of healthy donors, for treatment of Degenerative Disc Disease (DDD). The trial is based in previous results with autologous MSC (Orozco et al., Transplantation 92: 822-828; 2011). Here we propose a phase I-II trial, prospective, randomized, blinded, and controlled for the treatment DDD using MSV, a Good Manufacturing Practice (GMP)-compliant expanded bone marrow MSC (MSV, Investigational medicinal product Num. 10-134). The assay consists of two arms with 12 patients each one. Patients in the experimental arm will be given a single intra-discal transplantation of MSV (25 millions in 2 ml). Control patients will be infiltrated in the paravertebral muscles close to the lesion with 2 ml of 1% mepivacain. We shall follow the evolution of pain, disability and quality of life as well as disc fluid content by Magnetic Resonance Imaging (T2-calibrated).

Eligibility Criteria

Inclusion Criteria

  • Degenerative disease of one or two lumbar discs with predominant back pain after conservative treatment (physical and medical) for over 6 months.
  • Fibrous ring capable of holding the cell implantation, demonstrated by Magnetic resonance imaging (MRI) image (stages 2, 3 and 4 of Adams).
  • Decrease of disc height of more than 20% (radiographic measurement in side image).
  • Absence of spinal infection.
  • Haematological and biochemical analysis wit no significant alterations that contraindicates intervention.
  • The patient is able to understand the nature of the study.
  • Informed written consent of the patient.

Exclusion Criteria

  • Age over 75 or under 18 or legally dependent
  • Allergy to gentamicin, or to bovine, cattle or horse serum.
  • Congenital or acquired diseases leading to spine deformations that may upset cell application.
  • Spinal segmental instability, spinal canal stenosis, isthmus pathology and other conditions that may compromise the study
  • Modic III changes on MRI images (31).
  • Overweight with body mass index (mass in Kg/size in m2) greater than 35 (obesity grade II).
  • Pregnancy or breast-feeding
  • Neoplasia
  • Immunosuppression
  • Participation in another clinical trial or treatment with another investigational product within 30 days prior to inclusion in the study.
  • Other conditions that may, according to medical criteria, discourage participation in the study.
View full record on ClinicalTrials.gov →

Data sourced from ClinicalTrials.gov (NCT01860417). Outcome figures and adverse-event rates are extracted automatically from the registry's posted results and are provided for clinician reference, not as a substitute for the primary publication.

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