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Whey protein ranked first for lean body mass gains during supervised resistance trainingWhey protein ranks highest for building lean muscle mass

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Key Takeaway
Consider whey protein at 30-35 g/d for maximal lean body mass gains during resistance training.

This Bayesian network meta-analysis synthesized data from 36 RCTs involving 89 trial arms and 1,552 participants to evaluate various protein sources combined with supervised resistance training (RT). The analysis compared whey, casein, soy, pea, rice, milk, and mixed proteins against placebo for lean body mass (LBM) gains over periods of at least 6 weeks.

Whey protein was ranked first for LBM gains (SUCRA = 0.91), associated with an increase of 1.24 kg compared to placebo. Milk ranked second (SUCRA = 0.78, +1.18 kg), and casein ranked third (SUCRA = 0.72, +0.92 kg). A dose-response analysis for whey protein suggested a plateau at 30-35 g/d. In older adults (aged 65 years or older), the superiority of whey was narrower, with casein SUCRA increasing from 0.68 to 0.76.

Several limitations were noted, including low to very low evidence certainty for plant-based proteins due to wide credible intervals and an exploratory nature of the whey dose-response estimate. Additionally, ranking changes in older adults may be unstable as that subgroup analysis was based on only nine trials. Clinical application suggests whey at 30-35 g/d for maximum gains, while older adults might benefit from casein or mixed proteins.

How this fits prior evidence

This finding extends the previous evidence that whey protein combined with structured exercise supports muscle health in older adults and may improve sarcopenia outcomes when combined with vitamin D and epicatechin. This meta-analysis specifically ranks whey as the most effective source for lean body mass gains among several types, including milk and casein, while noting that plant-based proteins currently have lower evidence certainty.

If you are hitting the gym to build strength or gain muscle, choosing the right protein might matter more than you think. A large review of 36 different trials involving over 1,500 people looked at how different types of protein—like whey, milk, casein, and plant-based options—affected lean body mass when combined with resistance training.

The findings show that whey protein ranked first for increasing lean muscle mass compared to a placebo. Specifically, whey was associated with an average gain of 1.24 kg of lean mass. Milk followed in second place, while casein ranked third. The study also noted that for people aged 65 and older, the gap between whey and other proteins narrowed, suggesting that casein or mixed proteins could be very effective options for older adults.

While the results are promising for dairy-based proteins, the evidence for plant-based options like soy, pea, or rice was less certain. These proteins showed smaller effects in the study, and researchers noted that the data for them was not as strong. If you are looking to adjust your supplement routine, talk to a professional to see which protein fits your specific goals.

What this means for you:
Whey protein shows the highest gains for lean muscle mass when combined with resistance training.

Common questions

Which protein is best for gaining muscle?

Whey protein ranked first for increasing lean body mass in this study. It was associated with an average gain of 1.24 kg compared to a placebo. Milk and casein also showed positive results, ranking second and third respectively.

Are plant-based proteins effective for muscle growth?

The study found that plant-based proteins, such as soy, pea, or rice, had smaller effect estimates than dairy-based ones. However, the researchers noted that the evidence for these specific plant options was low to very low.

Is there a specific amount of whey protein I should take?

The study looked at how much whey protein people took and found a plateau in results at 30 to 35 grams per day. This means taking more than that amount did not lead to higher gains.

Study Details

Study typeMeta analysis
EvidenceLevel 1
PublishedAug 2026
View Original Abstract ↓
BackgroundProtein supplementation is frequently utilized in conjunction with resistance training for the purpose of enhancing muscle mass and strength. Nevertheless, the efficacy of different protein types, dosing thresholds, and population-specific effects is debatable.MethodsA systematic search was performed on PubMed, Web of Science, Scopus, SPORTDiscus, Cochrane CENTRAL, EMBASE databases through February 2026. RCTs comparing protein supplements (whey, casein, soy, pea, rice, milk, or blend) with placebo during supervised RT (≥6 weeks) were eligible. A Bayesian random-effects NMA was conducted using gemtc and JAGS. Dose–response curves were fitted with restricted cubic splines. The protocol was registered on PROSPERO (CRD420261340515).ResultsThirty-six RCTs (1,552 participants; 89 trial arms) formed a fully connected network of eight nodes. For LBM, whey protein ranked first (SUCRA = 0.91; MD vs. placebo: +1.24 kg, 95% CrI 0.61–1.87), followed by milk (SUCRA = 0.78; +1.18 kg) and casein (SUCRA = 0.72; +0.92 kg). The dose–response curve for whey indicated a plateau at 30–35 g/d. In older adults (≥65 years), the superiority of whey narrowed (SUCRA = 0.89 vs. 0.92 in younger adults), while casein’s SUCRA increased from 0.68 in younger adults to 0.76 in older adults. However, this subgroup analysis was based on only nine trials, and the observed ranking changes may be unstable. Model convergence was satisfactory (R̂̂ = 1.01; ESS = 3,420), and node-splitting detected no significant inconsistency (all p > 0.10).ConclusionAmong commonly available protein supplements, whey protein at approximately 30–35 g/d was associated with the largest gains in lean body mass when combined with RT, although this dose–response estimate is exploratory. Older adults may benefit from casein- or mixed-protein strategies as the advantage of whey is diminished in this population. Plant-based proteins showed smaller effect estimates than dairy-derived proteins, but the evidence certainty was low to very low and the credible intervals were wide, limiting firm conclusions.Systematic review registrationIdentifier: CRD420261340515.
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