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N/A Completed N=77 Randomized Single-blind Basic Science

The Influence of Physical Activity on the Gut Microbiome of Pre-Diabetic Adults

Obesity · Overweight · Overweight and Obesity · Prediabetes
Source: ClinicalTrials.gov NCT04931836 ↗
Enrolled (actual)
77
Serious AEs
0.0%
Results posted
Jun 2025
Primary outcomePrimary: Change in Shannon Index (Unitless) — -0.20; 0.013 units on a scale

Summary

The present study is a 100-participant randomized controlled 2-arm parallel trial that employs a metagenomic approach to examine how 8 weeks of supervised moderate-intensity treadmill walking exercise (MWE) for 30-45 min 3 times/week alters the gut microbiome, serum short chain fatty acids, and the cardiometabolic profile, body weight, and body composition of individuals 30-64 years old who have overweight or obesity and have prediabetes.

Outcome Measures

OutcomeResultp-value
PRIMARY
Change in Shannon Index (Unitless)
-0.20; 0.013
PRIMARY
Change in Serum Short Chain Fatty Acids (SCFA)
0.19; 0.08
SECONDARY
High-density Lipoprotein Cholesterol (HDL-C)
53.4; 51.7
SECONDARY
Triglycerides
147.2; 142.7
SECONDARY
Fasting Insulin
132.5; 134.8
SECONDARY
Fasting Glucose
105.0; 105.0
SECONDARY
C-reactive Protein (CRP)
4.6; 4.5
SECONDARY
Resting Systolic Blood Pressure (BP)
121; 120
SECONDARY
Body Composition
43.5; 43.6
SECONDARY
Cardiometabolic Risk Score (CMR)
-.01; -.04

Eligibility Criteria

Inclusion Criteria

  • Classified as overweight or obese with BMI 25.0-39.9 kg/m2.
  • Documentation* of a prediabetes diagnosis within one year of enrollment by physician or primary care provider based on lab tests showing a fasted blood glucose of 100-125 mg/dL, a 2-hour oral glucose tolerance test of 140-199 mg/dL, or an HbA1c level of 5.7%-6.4%76; OR a study screening lab value of HbA1C within the afore mentioned range.
  • Currently engaged in <100 min/week of physical activity - confirmed by questionnaire.
  • No exercise contraindications as assessed by the Physical Activity Readiness Questionnaire (PAR-Q)78-this Questionnaire involves seven "yes" or "no" questions regarding an individual's health status, with answering "yes" to any one of these questions requiring a prospective participant to acquire a written doctor's note stating they can safely participate in the trial's exercise intervention.
  • No self-reported physical/mental disabilities or gastrointestinal conditions.
  • No antibiotic usage within the last 45 days.
  • Stable weight over the last 6 months (<10% change).
  • Not currently pregnant, planning to become pregnant, or currently breastfeeding.
  • Willing to maintain current dietary and exercise habits, aside from any changes to be made per the study exercise protocol.
  • Note: Documentation can include either a print out or screen shot of the lab value illustrating eligibility, along with the date of the test and the participant's name. If a hard copy is provided, the date and name will be redacted.

Exclusion Criteria

  • Self-reported use of metformin and/or other medications that could interfere with the primary outcome.
  • History of bariatric surgery or a history of other medical interventions that would interfere with the primary outcome.
View full record on ClinicalTrials.gov →

Data sourced from ClinicalTrials.gov (NCT04931836). Outcome figures and adverse-event rates are extracted automatically from the registry's posted results and are provided for clinician reference, not as a substitute for the primary publication. Informational only — not medical advice.

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